SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

myosin IIIA

Synonyms

9030416P08Rik

Chromosome

Genetic Map Distance

15.15 cM

RefSeq Accessions

NM_148413; NP_680779

NCBI Entrez Gene

667663 at chr2:22149130-22473772 (mm9) / chr0:0-0 (mm10)

Ensembl Gene

ENSMUSG00000025716

VEGA Gene

OTTMUSG00000011499

MGI Gene

MGI:2183924

Mouse Protein (4)

UniProt ID	Description	Length (AA)
Q8K3H5	Myosin-IIIa	1613
A2ASW0	Myosin IIIA (Fragment)	683
B1AYY3	Myosin IIIA (Fragment)	753
Q9D336	Putative uncharacterized protein	207

Pfam Domains

Pkinase; Myosin_head; Hpr_kinase_C; IQ

Mouse alleles (3)

Allele Name	Allele Type
Myo3a^{tm1a(KOMP)Wtsi}	KOMP-CSD-Project
Myo3a^{tm1e(KOMP)Wtsi}	KOMP-CSD-Project
Myo3a^tm1.1Mckg	Targeted (knock-in)

Human Homolog

MYO3A (myosin IIIA)

Human synonyms

DFNB30

Human cytoband

10p11.1

Known deafness gene

DFNB30

Within mapped deafness loci

Associated Human Disease in OMIM

Deafness, Autosomal Recessive 30; DFNB30

Chicken homolog (1)

ENSGALG00000007647 (MYO3A) encoding ENSGALP00000012361

Gene Ontology

Biological Process (3)	sensory perception of sound; protein autophosphorylation; ATP catabolic process
Cellular Component (3)	filopodium; myosin complex; filamentous actin
Molecular Function (8)	microfilament motor activity; protein kinase activity; ATP binding; nucleotide binding; calmodulin binding; actin-dependent ATPase activity; ADP binding; plus-end directed microfilament motor activity

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	30	7	31	147	81	58
Cochlea GFP+ cells	0	15	321	185	99	NA
Utricle GFP- cells	0	2	0	0	0	62
Cochlea GFP- cells	0	0	0	0	0	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	6	GFP+	88	14.36	1.64e-9
Utricle	35	Cochlea	62	1.76	0.724
Embryonic	7	Postnatal	70	10.01	0.000374

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17367473	173.99 ± 1.37	22.60 ± 12.06 [25.73]	0.13	0.262

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	0	0	0	0
No bFGF	0	0	0	0
EGF	0	1	0	0

Analysis

Condition A	Condition B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	No bFGF	0.00	1
bFGF	EGF	0.00	0.31
No bFGF	EGF	3.56	0.684

Chicken Stereocilium Proteomics

Protein ID	ENSGALP00000012361
Symbol	MYO3A
Description	Myosin IIIA
log10 riBAQ in stereocilia (mean ± sd)	-5.52 ± 0.33
log10 riBAQ in utricle (mean ± sd)	-5.96 ± 0.35
Molecules per stereocilium (mean ± error with slope error)	4.40 ± 0.29
Corrected Molecules per stereocilium (mean ± sd)	4.19 ± 0.70
Stereocilium / Utricle ratio (mean ± sd)	2.71 ± 0.25
Enriched in the stereocilia (FDR < 0.05)	FALSE

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1431983_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	8.54	19.47	26.19	28.52	30.84	36.78
Replicate 1 Call	A	A	A	A	A	A
Replicate 2	4.36	32.09	48.47	10.96	17.17	20.42
Replicate 2 Call	A	A	A	A	A	A
Replicate 3	19.38	20.02	16.79	23.22	10.02	14.11
Replicate 3 Call	A	A	A	A	A	A
Replicate 4			9.87	27.37	21.3	11.44
Replicate 4 Call			A	A	A	A
Mean	10.76	23.86	25.33	22.52	19.83	20.69
Standard Deviation	7.75	7.13	16.81	8.03	8.69	11.37
Log2 Mean	3.17	4.54	4.42	4.4	4.2	4.22
Log2 Standard Deviation	1.08	0.41	0.97	0.64	0.68	0.74

Vestibular ganglion
Replicate 1	7.77	9.04	13.11	44.32	8.31	31.69
Replicate 1 Call	A	A	A	A	A	A
Replicate 2	8.23	24.02	32.53	13.91	19.61	32.77
Replicate 2 Call	A	A	A	A	A	A
Replicate 3	10.87	10.43	35.24	40.57	8.13	16.25
Replicate 3 Call	A	A	A	A	A	A
Mean	8.96	14.5	26.96	32.93	12.02	26.9
Standard Deviation	1.67	8.28	12.07	16.58	6.58	9.24
Log2 Mean	3.15	3.72	4.63	4.87	3.46	4.68
Log2 Standard Deviation	0.26	0.76	0.79	0.93	0.72	0.57

GENE VIEW

Myo3a