SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

myosin, heavy polypeptide 14

Synonyms

NMHC II-C; 2400004E04Rik

Chromosome

Genetic Map Distance

28.85 cM

RefSeq Accessions

NM_028021; NP_082297

NCBI Entrez Gene

71960 at chr7:51861189-51920873 (mm9) / chr7:44605803-44670913 (mm10)

Ensembl Gene

ENSMUSG00000030739

VEGA Gene

OTTMUSG00000017593

MGI Gene

MGI:1919210

Mouse Protein (4)

UniProt ID	Description	Length (AA)
Q6URW6	Myosin-14	2000
B3F3T1	Nonmuscle myosin II-C2	2033
D3Z5V8	Uncharacterized protein	121
Q7TQH2	Myh14 protein (Fragment)	603

Pfam Domains

Myosin_N; Myosin_head; IQ; Myosin_tail_1; ADIP; ADIP

Mouse alleles (4)

Allele Name	Allele Type
Myh14^{tm1a(EUCOMM)Wtsi}	EUCOMM-projects
Myh14^{tm1e(EUCOMM)Wtsi}	EUCOMM-projects
Myh14^tm1Rsad	Targeted (knock-out)
Myh14^tm2Rsad	Targeted (knock-in)

Human Homolog

MYH14 (myosin, heavy chain 14, non-muscle)

Human synonyms

DFNA4; MHC16; MYH17; myosin; NMHC II-C; NMHC-II-C; PNMHH

Human cytoband

19q13.33

Known deafness gene

DFNA4

Within mapped deafness loci

Associated Human Disease in OMIM

Deafness, Autosomal Dominant 4; DFNA4

Chicken homolog (0)

Gene Ontology

Biological Process (4)	actin filament-based movement; actin filament-based movement; regulation of cell shape; metabolic process
Cellular Component (5)	myosin complex; axon; cytoplasm; growth cone; stress fiber
Molecular Function (11)	ATP binding; actin filament binding; actin-dependent ATPase activity; microfilament motor activity; microfilament motor activity; actin-dependent ATPase activity; actin filament binding; actin binding; calmodulin binding; nucleotide binding; motor activity

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	581	7	150	90	15	359
Cochlea GFP+ cells	24	63	66	31	40	NA
Utricle GFP- cells	7	11	23	45	100	424
Cochlea GFP- cells	7	57	35	17	9	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	67	GFP+	130	1.93	0.252
Utricle	151	Cochlea	35	0.23	1
Embryonic	95	Postnatal	100	1.06	0.799

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17490328	28.86 ± 5.28	22.98 ± 4.54 [14.53]	0.80	0.554

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	1121	1164	1647	993
No bFGF	884	1086	1301	1635
EGF	188	198	250	212

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	993	No bFGF	1635	1.65	0.374
bFGF	993	EGF	212	0.21	5.8e-49
No bFGF	1635	EGF	212	0.13	4.47e-94

Chicken Stereocilium Proteomics

No chicken homolog has been identified for Myh14.

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1428835_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	423.05	395.04	690.59	1338.14	917.72	1399.24
Replicate 1 Call	A	M	P	M	M	P
Replicate 2	467.06	437.07	944.23	511.43	1324.51	541.68
Replicate 2 Call	A	A	P	A	P	A
Replicate 3	566.01	525.05	1040.83	1227.83	1301.4	2324.86
Replicate 3 Call	A	P	A	P	A	P
Replicate 4			859.44	984.47	1313.78	2610.49
Replicate 4 Call			P	P	M	P
Mean	485.37	452.39	883.77	1015.47	1214.35	1719.07
Standard Deviation	73.22	66.34	148.59	367.07	197.98	939.88
Log2 Mean	8.91	8.81	9.77	9.9	10.23	10.52
Log2 Standard Deviation	0.21	0.21	0.25	0.63	0.26	1.03

Vestibular ganglion
Replicate 1	554.63	582.47	535.68	507.28	921.39	1803.76
Replicate 1 Call	A	P	P	A	P	P
Replicate 2	509.67	521.63	679.78	730.91	1325.39	1556.42
Replicate 2 Call	A	P	M	A	P	P
Replicate 3	594.56	516.72	284.62	688.59	1051.49	1600.97
Replicate 3 Call	A	P	A	A	P	P
Mean	552.95	540.27	500.03	642.26	1099.42	1653.72
Standard Deviation	42.47	36.63	199.98	118.8	206.22	131.84
Log2 Mean	9.11	9.08	8.88	9.31	10.09	10.69
Log2 Standard Deviation	0.11	0.1	0.65	0.28	0.27	0.11

GENE VIEW

Myh14