SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

crystallin, mu

Synonyms

Chromosome

Genetic Map Distance

64.47 cM

RefSeq Accessions

NM_016669; NP_057878

NCBI Entrez Gene

12971 at chr7:127329898-127345502 (mm9) / chr7:120186384-120201988 (mm10)

Ensembl Gene

ENSMUSG00000030905

VEGA Gene

OTTMUSG00000027354

MGI Gene

MGI:102675

Mouse Protein (2)

UniProt ID	Description	Length (AA)	Predicted # TMs
O54983	Mu-crystallin homolog	313	0
Q3UPX0	Crystallin, mu	313	0

Pfam Domains

OCD_Mu_crystall

Mouse alleles (4)

Allele Name	Allele Type
Crym^{tm1e(KOMP)Wtsi}	KOMP-CSD-Project
Crym^{tm1a(KOMP)Wtsi}	KOMP-CSD-Project
Crym^{tm1(KOMP)Vlcg}	KOMP-Regeneron-Project
Crym^tm1Sasu	Targeted (knock-out)

Human Homolog

CRYM (crystallin, mu)

Human synonyms

DFNA40; THBP

Human cytoband

16p

Known deafness gene

DFNA40

Within mapped deafness loci

Associated Human Disease in OMIM

None

Chicken homolog (1)

ENSGALG00000002484 (CRYM) encoding ENSGALP00000003906

Gene Ontology

Biological Process (5)	oxidation-reduction process; thyroid hormone transport; negative regulation of transcription from RNA polymerase II promoter; sensory perception of sound; thyroid hormone metabolic process
Cellular Component (5)	cytoplasm; plasma membrane; mitochondrion; nucleus; nucleus
Molecular Function (8)	thyroid hormone binding; nucleotide binding; transcription corepressor activity; protein homodimerization activity; NADP binding; thiomorpholine-carboxylate dehydrogenase activity; oxidoreductase activity; hormone binding

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	117	192	327	165	384	111
Cochlea GFP+ cells	349	547	474	56	128	NA
Utricle GFP- cells	585	270	103	21	7	0
Cochlea GFP- cells	2918	1891	1507	765	438	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	773	GFP+	259	0.34	0.423
Utricle	190	Cochlea	907	4.77	1
Embryonic	859	Postnatal	320	0.37	0.31

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17495790	14.99 ± 0.59	15.67 ± 1.73 [12.19]	1.05	0.666

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	20	21	21	15
No bFGF	3	0	1	1
EGF	0	0	0	0

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	15	No bFGF	1	0.08	0.00143
bFGF	15	EGF	0	0.00	1.27e-12
No bFGF	1	EGF	0	0.00	0.126

Chicken Stereocilium Proteomics

The chicken protein was not reproducibly detected in the chicken stereocilia.

The chicken protein was not reproducibly detected in the chicken stereocilia.

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1416776_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	2246.05	2890.24	3273.7	4799.74	4976.42	1363.76
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	2240.29	3300.31	3816.71	5503.25	4100.34	843.17
Replicate 2 Call	P	P	P	P	P	A
Replicate 3	2362.28	3282.92	3263.41	4621.83	5797.3	896.7
Replicate 3 Call	P	P	P	P	P	P
Replicate 4			3819.46	3551.34	5713.33	1426.17
Replicate 4 Call			P	P	P	P
Mean	2282.87	3157.82	3543.32	4619.04	5146.85	1132.45
Standard Deviation	68.83	231.9	317.3	807.15	789.14	304.98
Log2 Mean	11.16	11.62	11.79	12.16	12.32	10.1
Log2 Standard Deviation	0.04	0.11	0.13	0.26	0.23	0.4

Vestibular ganglion
Replicate 1	1980.73	1955.35	1754.34	1796.26	2037.37	938.7
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	1936.4	1673.61	1485.31	2493.44	1902.63	1679.32
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	2046.77	2120.1	1448.99	1933.57	1787.07	1338.26
Replicate 3 Call	P	P	P	P	P	P
Mean	1987.97	1916.35	1562.88	2074.42	1909.02	1318.76
Standard Deviation	55.54	225.79	166.8	369.32	125.27	370.69
Log2 Mean	10.96	10.9	10.6	11	10.9	10.32
Log2 Standard Deviation	0.04	0.17	0.15	0.25	0.09	0.42

GENE VIEW

Crym