GENE VIEW

Tubb3

MOUSE GENE INFORMATION [PubMed]

Description

tubulin, beta 3 class III

Synonyms

3200002H15Rik; betaIII-tubulin; Tuj1

Chromosome

8

Genetic Map Distance

72.10 cM

RefSeq Accessions

NM_023279; NP_075768

NCBI Entrez Gene

22152 at chr8:125935464-125945910 (mm9) / chr8:123411564-123422010 (mm10)

Ensembl Gene

ENSMUSG00000062380

VEGA Gene

OTTMUSG00000027611

MGI Gene

Mouse Protein (3)

UniProt ID	Description	Length (AA)	Predicted # TMs
Q9ERD7	Tubulin beta-3 chain	450	0
D5MR34	Tubulin beta 3 (Fragment)	133	0
Q9CRT0	Putative uncharacterized protein (Fragment)	235	0

Pfam Domains

Tubulin; Tubulin_C

Mouse alleles (2)

Allele Name	Allele Type
Tubb3^{tm1a(KOMP)Wtsi}	KOMP-CSD-Project
Tubb3^tm1.1Ece	Targeted (knock-in)

Human Homolog

TUBB3 (tubulin, beta 3 class III)

Human synonyms

beta-4; CDCBM; CFEOM3A; TUBB4

Human cytoband

16q24.3

Known deafness gene

No

Within mapped deafness loci

No

Associated Human Disease in OMIM

Fibrosis of Extraocular Muscles, Congenital, 3a, with or without Extraocular Involvement; CFEOM3A [Feom3 Locus]

Chicken homolog (0)

Biological Process (8)	GTP catabolic process; axon guidance; axon guidance; mitosis; neuron differentiation; protein polymerization; microtubule-based process; microtubule-based movement
Cellular Component (10)	microtubule; microtubule; cytoskeleton; cytoplasm; cytoplasm; intracellular; axon; axon; microtubule cytoskeleton; protein complex
Molecular Function (6)	peptide binding; nucleotide binding; GTPase activity; structural molecule activity; GTP binding; GTP binding

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	312	53	12	6	83	19
Cochlea GFP+ cells	7353	104	2	7	9	NA
Utricle GFP- cells	26	20	39	0	1	0
Cochlea GFP- cells	70	114	8	54	25	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	32	GFP+	724	22.03	4.56e-8
Utricle	47	Cochlea	775	16.15	0.00874
Embryonic	1006	Postnatal	19	0.02	8.61e-8

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17506631	6.15 ± 0.73	7.88 ± 3.30 [15.13]	1.28	0.509

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	391	268	288	267
No bFGF	966	964	1108	1562
EGF	25706	25225	33211	28047

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	267	No bFGF	1562	5.83	7.7e-7
bFGF	267	EGF	28047	104.66	5.32e-90
No bFGF	1562	EGF	28047	17.95	7.43e-80

Chicken Stereocilium Proteomics

No chicken homolog has been identified for Tubb3.

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1415978_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	2311.63	1130.8	578.49	215.62	78.46	12.91
Replicate 1 Call	P	P	P	P	P	A
Replicate 2	2302.16	1690.68	373.09	59.53	194.11	14.6
Replicate 2 Call	P	P	P	P	P	A
Replicate 3	2356.32	1556.99	460.31	275.89	9.05	70.87
Replicate 3 Call	P	P	P	P	A	P
Replicate 4			327.13	282.73	12.82	104.07
Replicate 4 Call			P	P	A	P
Mean	2323.37	1459.49	434.76	208.44	73.61	50.61
Standard Deviation	28.93	292.4	110.6	103.75	86.42	44.67
Log2 Mean	11.18	10.49	8.73	7.47	5.19	5.1
Log2 Standard Deviation	0.02	0.31	0.36	1.07	2.11	1.55

Vestibular ganglion
Replicate 1	1754.47	786.69	74.97	75.96	91.07	408.37
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	1819.66	972.82	225.26	109.19	168.43	436.43
Replicate 2 Call	P	P	P	M	P	P
Replicate 3	1630.23	761.48	57.47	258.97	179.41	400.49
Replicate 3 Call	P	P	P	P	P	P
Mean	1734.79	840.33	119.23	148.04	146.3	415.1
Standard Deviation	96.24	115.43	92.24	97.49	48.15	18.89
Log2 Mean	10.76	9.71	6.63	7.01	7.13	8.7
Log2 Standard Deviation	0.08	0.19	1.04	0.91	0.54	0.07