SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

succinate-Coenzyme A ligase, ADP-forming, beta subunit

Synonyms

4930547K18Rik

Chromosome

Genetic Map Distance

39.06 cM

RefSeq Accessions

NP_035636; XM_127733

NCBI Entrez Gene

20916 at chr14:73952593-73995949 (mm9) / chr14:73552786-73596144 (mm10)

Ensembl Gene

ENSMUSG00000022110

VEGA Gene

OTTMUSG00000035022

MGI Gene

MGI:1306775

Mouse Protein (5)

UniProt ID	Description	Length (AA)
Q9Z2I9	Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial	463
Q05BC6	Sucla2 protein	224
Q3U6C7	Putative uncharacterized protein (Fragment)	470
Q3UCC6	Putative uncharacterized protein (Fragment)	470
Q9CTE2	Putative uncharacterized protein (Fragment)	98

Pfam Domains

HipA_N; ATP-grasp_2; Ligase_CoA

Mouse alleles (3)

Allele Name	Allele Type
Sucla2^{tm1e(EUCOMM)Wtsi}	EUCOMM-projects
Sucla2^{tm1e(EUCOMM)Hmgu}	EUCOMM-projects
Sucla2^{tm1a(EUCOMM)Wtsi}	EUCOMM-projects

Human Homolog

SUCLA2 (succinate-CoA ligase, ADP-forming, beta subunit)

Human synonyms

A-BETA; MTDPS5; SCS-betaA

Human cytoband

13q12.2-q13.3

Known deafness gene

Within mapped deafness loci

Associated Human Disease in OMIM

Mitochondrial DNA Depletion Syndrome 3 (hepatocerebral Type); MTDPS3

Mitochondrial DNA Depletion Syndrome 2 (myopathic Type); MTDPS2 [Mitochondrial DNA Depletion Myopathy, Tk2-Related]

Mitochondrial DNA Depletion Syndrome 5 (encephalomyopathic with Methylmalonic Aciduria); MTDPS5 [Mitochondrial DNA Depletion Syndrome, Encephalomyopathic Form, with Methylmalonic Aciduria, Autosomal Recessive, Sucla2-Related]

Chicken homolog (2)

ENSGALG00000016993 (Q5F3B9_CHICK) encoding ENSGALP00000027401

ENSGALG00000016993 (Q5F3B9_CHICK) encoding ENSGALP00000035707

Gene Ontology

Biological Process (4)	metabolic process; tricarboxylic acid cycle; succinyl-CoA metabolic process; succinate metabolic process
Cellular Component (4)	mitochondrion; mitochondrion; mitochondrion; mitochondrion
Molecular Function (9)	catalytic activity; succinate-CoA ligase (ADP-forming) activity; ATP binding; ATP binding; ligase activity; ligase activity; metal ion binding; nucleotide binding; succinate-CoA ligase activity

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	477	615	835	290	1144	333
Cochlea GFP+ cells	593	755	930	1039	1083	NA
Utricle GFP- cells	629	935	930	795	1070	613
Cochlea GFP- cells	615	683	690	887	843	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	790	GFP+	736	0.93	0.977
Utricle	722	Cochlea	812	1.12	1
Embryonic	663	Postnatal	820	1.24	1

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17301899	99.08 ± 2.46	93.54 ± 3.13 [97.34]	0.94	0.501

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	473	491	387	484
No bFGF	625	598	528	494
EGF	509	508	457	492

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	484	No bFGF	494	1.02	1
bFGF	484	EGF	492	1.01	0.977
No bFGF	494	EGF	492	1.00	1

Chicken Stereocilium Proteomics

Protein ID	ENSGALP00000027401
Symbol	SUCLA2
Description	Succinate-CoA ligase, ADP-forming, beta subunit
log10 riBAQ in stereocilia (mean ± sd)	-4.66 ± 0.30
log10 riBAQ in utricle (mean ± sd)	-3.64 ± 0.07
Molecules per stereocilium (mean ± error with slope error)	32.07 ± 2.25
Corrected Molecules per stereocilium (mean ± sd)	-36.94 ± 87.22
Stereocilium / Utricle ratio (mean ± sd)	0.09 ± 0.01
Enriched in the stereocilia (FDR < 0.05)	FALSE

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1430402_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	6.39	42.37	16.88	37.4	25.91	21.3
Replicate 1 Call	A	P	A	P	P	P
Replicate 2	11.9	34.57	25.26	21.88	12.58	30.69
Replicate 2 Call	A	P	A	A	P	A
Replicate 3	28.25	34.97	18.92	27.51	33.23	32.71
Replicate 3 Call	A	P	A	P	P	A
Replicate 4			30.52	23.78	44.85	33.08
Replicate 4 Call			A	P	P	P
Mean	15.51	37.3	22.9	27.64	29.14	29.45
Standard Deviation	11.37	4.39	6.21	6.91	13.52	5.53
Log2 Mean	3.69	5.21	4.48	4.76	4.72	4.86
Log2 Standard Deviation	1.08	0.16	0.39	0.34	0.78	0.3

Vestibular ganglion
Replicate 1	32.67	27.85	11	4.92	42.46	40.76
Replicate 1 Call	P	P	A	A	M	A
Replicate 2	28.95	25.38	34.8	27.25	19.4	29.78
Replicate 2 Call	P	P	P	P	A	P
Replicate 3	22.17	21.03	34.81	29.25	13.08	25.67
Replicate 3 Call	P	P	A	A	P	P
Mean	27.93	24.75	26.87	20.47	24.98	32.07
Standard Deviation	5.32	3.45	13.74	13.51	15.46	7.8
Log2 Mean	4.79	4.62	4.57	3.98	4.47	4.98
Log2 Standard Deviation	0.29	0.21	0.96	1.46	0.86	0.34

Probe set ID: 1452206_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	6886.88	6161.4	5138.17	5848.29	5818.78	4574.91
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	7016.66	7495.64	3555.68	4980.78	6540.14	4783.17
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	7387.36	7705.21	4300.22	5774.92	6131.54	5529.38
Replicate 3 Call	P	P	P	P	P	P
Replicate 4			3204.7	5881.29	5471.07	5475.22
Replicate 4 Call			P	P	P	P
Mean	7096.97	7120.75	4049.69	5621.32	5990.38	5090.67
Standard Deviation	259.72	837.4	857.44	429.33	455.08	483.36
Log2 Mean	12.79	12.79	11.96	12.45	12.55	12.31
Log2 Standard Deviation	0.05	0.18	0.3	0.11	0.11	0.14

Vestibular ganglion
Replicate 1	7463.22	7082.85	7319.39	8271.78	8512.57	8974.33
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	7241.27	7354.22	7231.62	7472.93	8595.09	8780.67
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	7119.49	7024.63	7157.3	7668.86	8325.4	9353.97
Replicate 3 Call	P	P	P	P	P	P
Mean	7274.66	7153.9	7236.1	7804.52	8477.69	9036.32
Standard Deviation	174.28	175.91	81.14	416.35	138.19	291.63
Log2 Mean	12.83	12.8	12.82	12.93	13.05	13.14
Log2 Standard Deviation	0.03	0.04	0.02	0.08	0.02	0.05

GENE VIEW

Sucla2