GENE VIEW

MOUSE GENE INFORMATION [PubMed]
Description polyribonucleotide nucleotidyltransferase 1
Synonyms PNPase; 1200003F12Rik; polynucleotide phosphorylase
Chromosome 11
Genetic Map Distance 16.46 cM
RefSeq Accessions NM_027869; NP_082145
NCBI Entrez Gene 71701 at chr11:29030751-29061828 (mm9) / chr11:29130663-29162068 (mm10)
Ensembl Gene ENSMUSG00000020464
VEGA Gene OTTMUSG00000005318
MGI Gene MGI:1918951
Mouse Protein (4)
UniProt ID Description Length (AA) Predicted # TMs
Q8K1R3 Polyribonucleotide nucleotidyltransferase 1, mitochondrial 783 0
Q3TN29 Putative uncharacterized protein 783 0
Q3TST0 Putative uncharacterized protein 783 0
Q3UNL5 Putative uncharacterized protein 783 0
Pfam Domains RNase_PH; RNase_PH_C; PNPase; RNase_PH; RNase_PH_C; KH_1; S1
Mouse alleles (5)
Allele Name Allele Type
Pnpt1tm1a(KOMP)Wtsi KOMP-CSD-Project
Pnpt1tm1e(KOMP)Wtsi KOMP-CSD-Project
Pnpt1tm2a(KOMP)Wtsi KOMP-CSD-Project
Pnpt1tm1.1Teit Targeted (knock-out)
Pnpt1tm1Teit Targeted (Floxed/Frt)
Human Homolog PNPT1 (polyribonucleotide nucleotidyltransferase 1)
Human synonyms old-35; OLD35; PNPASE
Human cytoband 2p15
Known deafness gene DFNB70
Within mapped deafness loci No
Associated Human Disease in OMIM
None
Chicken homolog (1)
ENSGALG00000007935 (PNPT1) encoding ENSGALP00000012877
Gene Ontology
Biological Process (28) RNA polyadenylation; negative regulation of growth; protein homooligomerization; positive regulation of mRNA catabolic process; RNA processing; protein homotrimerization; nuclear polyadenylation-dependent mRNA catabolic process; mitotic cell cycle arrest; positive regulation of miRNA catabolic process; regulation of cellular respiration; regulation of cellular respiration; regulation of cellular senescence; transport; mRNA processing; positive regulation of mitochondrial RNA catabolic process; mitochondrial mRNA catabolic process; RNA catabolic process; mitochondrial mRNA catabolic process; mitochondrial RNA 5'-end processing; mitochondrial RNA 3'-end processing; mRNA polyadenylation; mRNA catabolic process; mitochondrion morphogenesis; mitochondrion morphogenesis; cellular response to oxidative stress; cellular response to interferon-beta; RNA import into mitochondrion; rRNA import into mitochondrion
Cellular Component (10) mitochondrion; mitochondrion; mitochondrion; mitochondrion; mitochondrial intermembrane space; mitochondrial intermembrane space; cytoplasm; plasma membrane; mitochondrial degradosome; membrane
Molecular Function (12) RNA binding; RNA binding; 3'-5'-exoribonuclease activity; polyribonucleotide nucleotidyltransferase activity; hydrolase activity; nucleotidyltransferase activity; nuclease activity; exonuclease activity; poly(U) RNA binding; transferase activity; poly(G) RNA binding; miRNA binding
FACS-Sorted Hair Cells
Data | Chart
E16a
E16b
P0
P4
P7
P16
Utricle GFP+ cells
125
49
51
104
84
65
Cochlea GFP+ cells
97
131
160
75
69
NA
Utricle GFP- cells
121
222
153
74
103
78
Cochlea GFP- cells
149
204
290
317
100
NA
Analysis
Condition A
Mean A
Condition B
Mean B
Fold Change (B/A)
False Discovery Rate (FDR)
GFP-
165
GFP+
92
0.56
0.866
Utricle
102
Cochlea
159
1.55
1
Embryonic
137
Postnatal
123
0.90
1
Adult Cochlear Inner and Outer Hair Cells
Probeset ID IHC (mean ± sd) OHC (mean ± sd) [OHC replicate] Fold Change (OHC/IHC) False Discovery Rate (FDR)
17247962 32.03 ± 2.03 21.36 ± 7.32 [21.98] 0.67 0.554
Otic Progenitor Cells
Data
Growth Factor
Replicate 1
Replicate 2
Replicate 3
Mean
bFGF
378
326
368
404
No bFGF
335
291
305
303
EGF
291
345
291
309
Analysis
Condition A
Mean A
Condition B
Mean B
Fold Change (B/A)
False Discovery Rate (FDR)
bFGF
404
No bFGF
303
0.75
1
bFGF
404
EGF
309
0.76
0.0495
No bFGF
303
EGF
309
1.02
0.976
Chicken Stereocilium Proteomics
Protein ID ENSGALP00000012877
Symbol PNPT1
Description Polyribonucleotide nucleotidyltransferase 1
log10 riBAQ in stereocilia (mean ± sd) ND
log10 riBAQ in utricle (mean ± sd) -4.63 ± 0.22
Molecules per stereocilium (mean ± error with slope error) ND
Corrected Molecules per stereocilium (mean ± sd) ND
Stereocilium / Utricle ratio (mean ± sd) UTR only
Enriched in the stereocilia (FDR < 0.05) NA
Auditory and Vestibular Ganglion Neurons
Probe set ID: 1444288_at
Age E12 E13 E16 P0 P6 P15
Spiral ganglion
Replicate 1 359.31 137.71 502.65 355.9 313.42 199.23
Replicate 1 Call P P P P P P
Replicate 2 378.65 188.38 635.5 338.64 329.51 225.44
Replicate 2 Call P P P P P P
Replicate 3 345.33 260.44 409.96 335.07 423.59 284.04
Replicate 3 Call P P P P P P
Replicate 4 745.09 269.28 337.68 333.24
Replicate 4 Call P P P P
Mean 361.1 195.51 573.3 324.72 351.05 260.49
Standard Deviation 16.73 61.67 147.25 38.06 49.4 60.08
Log2 Mean 8.5 7.56 9.13 8.34 8.45 8
Log2 Standard Deviation 0.07 0.46 0.38 0.18 0.19 0.33
 
Vestibular ganglion
Replicate 1 419.42 321.45 420.35 218.97 161.43 90.33
Replicate 1 Call P P P P A P
Replicate 2 452.16 367.59 318.51 289.87 234.1 120.46
Replicate 2 Call P P P P P P
Replicate 3 430.53 413.92 211.61 289.1 181.51 108.11
Replicate 3 Call P P P P P P
Mean 434.04 367.65 316.82 265.98 192.35 106.3
Standard Deviation 16.65 46.24 104.38 40.71 37.53 15.15
Log2 Mean 8.76 8.51 8.25 8.04 7.57 6.72
Log2 Standard Deviation 0.06 0.18 0.5 0.23 0.27 0.21
Probe set ID: 1452676_a_at
Age E12 E13 E16 P0 P6 P15
Spiral ganglion
Replicate 1 1121.96 1711.22 950.27 987.85 1060.78 1155
Replicate 1 Call P P P P P P
Replicate 2 1168.8 1718.67 711.27 850.35 1070.58 871.14
Replicate 2 Call P P P P P P
Replicate 3 1321.87 1977.34 701.35 806.12 1513.54 793.01
Replicate 3 Call P P P P P P
Replicate 4 599.88 944.98 925.69 770.46
Replicate 4 Call A P P P
Mean 1204.21 1802.41 740.69 897.33 1142.65 897.4
Standard Deviation 104.55 151.54 148.51 83.65 255.95 177.07
Log2 Mean 10.23 10.81 9.51 9.8 10.13 9.79
Log2 Standard Deviation 0.12 0.12 0.28 0.13 0.3 0.27
 
Vestibular ganglion
Replicate 1 1091.36 1651.24 1974.81 2348.48 3095.3 2238.79
Replicate 1 Call P P P P P P
Replicate 2 1093.49 1643.45 1947.29 1968.53 2751.28 3031.53
Replicate 2 Call P P P P P P
Replicate 3 1067.58 1732.65 2291.52 1723.67 2719.76 3025.83
Replicate 3 Call P P P P P P
Mean 1084.14 1675.78 2071.21 2013.56 2855.45 2765.38
Standard Deviation 14.38 49.4 191.29 314.83 208.32 456.05
Log2 Mean 10.08 10.71 11.01 10.96 11.48 11.42
Log2 Standard Deviation 0.02 0.04 0.13 0.22 0.1 0.25
Probe set ID: 1452677_at
Age E12 E13 E16 P0 P6 P15
Spiral ganglion
Replicate 1 519.52 1105.13 705.1 914.59 745.36 142.38
Replicate 1 Call P P P P P A
Replicate 2 544.56 1344.79 267.42 811.47 856.29 337.98
Replicate 2 Call P P P P P A
Replicate 3 653.95 979.3 569.23 976.13 1682.45 722.09
Replicate 3 Call P P P P P P
Replicate 4 195.84 951.72 767.26 200.84
Replicate 4 Call P P P A
Mean 572.68 1143.07 434.4 913.48 1012.84 350.82
Standard Deviation 71.49 185.68 242.38 72.56 448.98 260.73
Log2 Mean 9.15 10.15 8.57 9.83 9.9 8.18
Log2 Standard Deviation 0.18 0.23 0.88 0.12 0.55 1.02
 
Vestibular ganglion
Replicate 1 669.86 1026.71 112.72 107.21 188.72 1346.2
Replicate 1 Call P P A A A P
Replicate 2 650.55 1253.6 348.99 137.27 1218.04 1390.61
Replicate 2 Call P P P A P P
Replicate 3 595.81 1129.18 1489.67 101.92 1722 177.57
Replicate 3 Call P P P A P P
Mean 638.74 1136.5 650.46 115.47 1042.92 971.46
Standard Deviation 38.41 113.62 736.32 19.07 781.5 687.89
Log2 Mean 9.32 10.15 8.6 6.84 9.52 9.44
Log2 Standard Deviation 0.09 0.14 1.87 0.23 1.72 1.7