SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

minichromosome maintenance deficient 2 mitotin (S. cerevisiae)

Synonyms

BM28; CDCL1; Mcmd2

Chromosome

Genetic Map Distance

39.64 cM

RefSeq Accessions

NM_008564; NP_032590

NCBI Entrez Gene

17216 at chr6:88833468-88848774 (mm9) / chr6:88883474-88898780 (mm10)

Ensembl Gene

ENSMUSG00000002870

VEGA Gene

MGI Gene

MGI:105380

Mouse Protein (3)

UniProt ID	Description	Length (AA)
P97310	DNA replication licensing factor MCM2	904
Q3UJN1	Putative uncharacterized protein	913
Q3UK39	Putative uncharacterized protein	904

Pfam Domains

MCM2_N; MCM

Mouse alleles (4)

Allele Name	Allele Type
Mcm2^{tm1a(EUCOMM)Wtsi}	EUCOMM-projects
Mcm2^{Gt(AB0178)Wtsi}	Gene trapped
Mcm2^{tm1(cre/ERT2)Scpr}	Targeted (knock-in)
Mcm2^tm2Scpr	Targeted (Reporter)

Human Homolog

MCM2 (minichromosome maintenance complex component 2)

Human synonyms

BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN

Human cytoband

3q21

Known deafness gene

Within mapped deafness loci

DFNB15

Associated Human Disease in OMIM

None

Chicken homolog (1)

ENSGALG00000006037 (Q5ZLZ1_CHICK) encoding ENSGALP00000009709

Gene Ontology

Biological Process (7)	cell cycle; DNA-dependent DNA replication initiation; DNA-dependent DNA replication initiation; DNA unwinding involved in replication; nucleosome assembly; DNA replication; DNA replication
Cellular Component (5)	nucleus; nucleus; nuclear origin of replication recognition complex; chromatin; MCM complex
Molecular Function (10)	hydrolase activity; nucleotide binding; helicase activity; DNA replication origin binding; DNA binding; DNA binding; metal ion binding; ATP binding; ATP binding; histone binding

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	112	24	8	13	3	6
Cochlea GFP+ cells	98	102	30	17	6	NA
Utricle GFP- cells	103	99	39	120	24	0
Cochlea GFP- cells	151	72	73	33	8	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	66	GFP+	38	0.58	0.867
Utricle	46	Cochlea	59	1.28	1
Embryonic	95	Postnatal	27	0.29	0.137

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17468798	21.17 ± 2.18	19.92 ± 0.61 [20.39]	0.94	0.701
17549538	27.22 ± 9.05	21.92 ± 3.44 [29.82]	0.81	0.7
17549690	27.22 ± 9.05	21.92 ± 3.44 [29.82]	0.81	0.7

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	4461	4353	5378	4885
No bFGF	2841	2933	3357	4376
EGF	4182	3977	4639	4266

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	4885	No bFGF	4376	0.90	1
bFGF	4885	EGF	4266	0.87	0.221
No bFGF	4376	EGF	4266	0.97	0.839

Chicken Stereocilium Proteomics

The chicken protein was not reproducibly detected in the chicken stereocilia.

The chicken protein was not reproducibly detected in the chicken stereocilia.

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1434079_s_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	3087.48	2652.91	1137.05	1831.35	1972.1	1829.99
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	3018.31	2669.79	1515.92	2977.82	2024.66	2095.33
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	2981.57	2650.75	1109.27	1152.69	1826.54	1286.41
Replicate 3 Call	P	P	P	P	P	P
Replicate 4			2024.91	885.56	2422.47	1752.5
Replicate 4 Call			P	P	P	P
Mean	3029.12	2657.82	1446.79	1711.86	2061.44	1741.06
Standard Deviation	53.78	10.43	427.73	933.16	254.86	336.78
Log2 Mean	11.56	11.38	10.45	10.58	11	10.74
Log2 Standard Deviation	0.03	0.01	0.41	0.77	0.17	0.3

Vestibular ganglion
Replicate 1	2684.54	2471.45	2547.56	1889.51	1278.88	939.44
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	2773.96	2475.87	2493.95	1879.7	1901.14	840.33
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	2770.04	2342.5	2181.1	1731.4	1863.59	868.01
Replicate 3 Call	P	P	P	P	P	P
Mean	2742.85	2429.94	2407.54	1833.54	1681.2	882.59
Standard Deviation	50.53	75.76	197.92	88.59	348.93	51.14
Log2 Mean	11.42	11.25	11.23	10.84	10.69	9.78
Log2 Standard Deviation	0.03	0.05	0.12	0.07	0.32	0.08

Probe set ID: 1448777_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	238.56	265.61	113.61	129.35	145.3	168.86
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	219.46	266.67	148.12	320.71	216.96	193.11
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	255.2	305.67	129.91	83.52	138.39	119.21
Replicate 3 Call	P	P	P	P	P	P
Replicate 4			182.8	82.27	212.85	125.47
Replicate 4 Call			P	P	P	P
Mean	237.74	279.32	143.61	153.96	178.38	151.66
Standard Deviation	17.88	22.83	29.69	113.3	42.31	35.37
Log2 Mean	7.89	8.12	7.14	7.02	7.45	7.22
Log2 Standard Deviation	0.11	0.12	0.29	0.92	0.35	0.34

Vestibular ganglion
Replicate 1	181.05	266.69	201.61	77.11	62.37	54.63
Replicate 1 Call	P	P	P	A	A	P
Replicate 2	217.92	236.98	213.39	78.62	99.22	48.69
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	168.45	169.54	119.39	102.72	98.01	63.42
Replicate 3 Call	P	P	P	P	P	P
Mean	189.14	224.4	178.13	86.15	86.53	55.58
Standard Deviation	25.71	49.78	51.21	14.37	20.93	7.41
Log2 Mean	7.55	7.78	7.43	6.42	6.4	5.79
Log2 Standard Deviation	0.19	0.34	0.46	0.23	0.38	0.19

GENE VIEW

Mcm2