GENE VIEW

MOUSE GENE INFORMATION [PubMed]
Description histidyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms Harsl; 4631412B19Rik; HARSR; HO3
Chromosome 18
Genetic Map Distance 19.46 cM
RefSeq Accessions NM_080636; NP_542367
NCBI Entrez Gene 70791 at chr18:36942934-36952216 (mm9) / chr18:36783232-36792562 (mm10)
Ensembl Gene ENSMUSG00000019143
VEGA Gene OTTMUSG00000033867
MGI Gene MGI:1918041
Mouse Protein (2)
UniProt ID Description Length (AA) Predicted # TMs
Q99KK9 Probable histidyl-tRNA synthetase, mitochondrial 505 0
Q99KH0 Hars2 protein (Fragment) 100 0
Pfam Domains tRNA-synt_2b; HGTP_anticodon
Mouse alleles (0)
Human Homolog HARS2 (histidyl-tRNA synthetase 2, mitochondrial (putative))
Human synonyms HARSL; HARSR; HO3
Human cytoband 5q31.3
Known deafness gene No
Within mapped deafness loci DFNA42; DFNA54; DFNB60
Associated Human Disease in OMIM
None
Chicken homolog (3)
ENSGALG00000000794 (Q5ZK86_CHICK) encoding ENSGALP00000001150
ENSGALG00000000794 (Q5ZK86_CHICK) encoding ENSGALP00000040480
ENSGALG00000024399 (HARS2) encoding ENSGALP00000040486
Gene Ontology
Biological Process (3) histidyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; translation
Cellular Component (2) mitochondrion; cytoplasm
Molecular Function (9) ATP binding; histidine-tRNA ligase activity; histidine-tRNA ligase activity; nucleotide binding; nucleotide binding; ligase activity; aminoacyl-tRNA ligase activity; aminoacyl-tRNA ligase activity; ATP binding
FACS-Sorted Hair Cells
Data | Chart
E16a
E16b
P0
P4
P7
P16
Utricle GFP+ cells
138
62
46
67
51
65
Cochlea GFP+ cells
71
100
82
91
54
NA
Utricle GFP- cells
111
61
90
19
52
31
Cochlea GFP- cells
116
60
69
137
105
NA
Analysis
Condition A
Mean A
Condition B
Mean B
Fold Change (B/A)
False Discovery Rate (FDR)
GFP-
77
GFP+
75
0.97
0.946
Utricle
66
Cochlea
88
1.34
1
Embryonic
90
Postnatal
69
0.76
0.998
Adult Cochlear Inner and Outer Hair Cells
Probeset ID IHC (mean ± sd) OHC (mean ± sd) [OHC replicate] Fold Change (OHC/IHC) False Discovery Rate (FDR)
17349774 35.70 ± 1.08 33.23 ± 1.19 [34.75] 0.93 0.501
Otic Progenitor Cells
Data
Growth Factor
Replicate 1
Replicate 2
Replicate 3
Mean
bFGF
830
832
639
711
No bFGF
895
857
732
746
EGF
773
735
614
707
Analysis
Condition A
Mean A
Condition B
Mean B
Fold Change (B/A)
False Discovery Rate (FDR)
bFGF
711
No bFGF
746
1.05
1
bFGF
711
EGF
707
0.99
1
No bFGF
746
EGF
707
0.95
0.835
Chicken Stereocilium Proteomics
The chicken protein was not reproducibly detected in the chicken stereocilia.
Auditory and Vestibular Ganglion Neurons
Probe set ID: 1419158_a_at
Age E12 E13 E16 P0 P6 P15
Spiral ganglion
Replicate 1 1088.27 1127.01 814.37 497.09 387.51 538.33
Replicate 1 Call P P P A A A
Replicate 2 1179.67 1226.59 417.82 446.01 462.36 351.52
Replicate 2 Call P P A A A A
Replicate 3 1222.52 1317.48 397.19 431.92 674.4 323.24
Replicate 3 Call P P A A A A
Replicate 4 311.48 393.93 483.57 271.66
Replicate 4 Call A A A A
Mean 1163.49 1223.69 485.22 442.24 501.96 371.19
Standard Deviation 68.57 95.27 224.22 42.67 122.12 116.23
Log2 Mean 10.18 10.25 8.82 8.78 8.94 8.49
Log2 Standard Deviation 0.09 0.11 0.59 0.14 0.33 0.42
 
Vestibular ganglion
Replicate 1 1113.02 977.93 692.34 856.09 544.91 957.16
Replicate 1 Call P P A A A P
Replicate 2 1098.96 1171.36 816.58 509.83 805.68 947.98
Replicate 2 Call P P A A A P
Replicate 3 986.51 1083.8 807.26 635.51 793.53 953.93
Replicate 3 Call P P A A A P
Mean 1066.16 1077.7 772.06 667.14 714.71 953.02
Standard Deviation 69.34 96.86 69.2 175.28 147.17 4.66
Log2 Mean 10.06 10.07 9.59 9.35 9.46 9.9
Log2 Standard Deviation 0.1 0.13 0.13 0.38 0.32 0.01
Probe set ID: 1449371_at
Age E12 E13 E16 P0 P6 P15
Spiral ganglion
Replicate 1 700.72 775.34 954.62 861.07 531.22 740.12
Replicate 1 Call P P P P P P
Replicate 2 708.65 790.08 787.23 625.4 545.07 271.34
Replicate 2 Call P P P P P P
Replicate 3 821.27 901.83 739.17 866.36 565.71 275.88
Replicate 3 Call P P P P P P
Replicate 4 636.37 839.1 456.81 396.8
Replicate 4 Call P P P P
Mean 743.55 822.42 779.35 797.98 524.7 421.04
Standard Deviation 67.43 69.17 132.71 115.66 47.43 220.52
Log2 Mean 9.53 9.68 9.59 9.63 9.03 8.59
Log2 Standard Deviation 0.13 0.12 0.24 0.23 0.14 0.68
 
Vestibular ganglion
Replicate 1 727.63 1145.39 672.11 759.5 569.47 836.7
Replicate 1 Call P P P P P P
Replicate 2 688.78 1199.7 929.9 521.19 549.19 942.6
Replicate 2 Call P P P P P P
Replicate 3 665.59 1258.47 971.15 728.87 551.55 822.25
Replicate 3 Call P P P P P P
Mean 694 1201.19 857.72 669.85 556.74 867.18
Standard Deviation 31.35 56.55 162.06 129.65 11.09 65.71
Log2 Mean 9.44 10.23 9.73 9.37 9.12 9.76
Log2 Standard Deviation 0.06 0.07 0.29 0.3 0.03 0.11