SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2

Synonyms

Dip3b

Chromosome

Genetic Map Distance

41.29 cM

RefSeq Accessions

NM_145220; NP_660255

NCBI Entrez Gene

216190 at chr10:83062778-83111409 (mm9) / chr10:83600033-83648882 (mm10)

Ensembl Gene

ENSMUSG00000020263

VEGA Gene

OTTMUSG00000027828

MGI Gene

MGI:2384914

Mouse Protein (7)

UniProt ID	Description	Length (AA)
Q8K3G9	DCC-interacting protein 13-beta	662
D3Z340	Uncharacterized protein	216
D6RFR7	Uncharacterized protein	117
Q3TB15	Putative uncharacterized protein	662
Q3TGG0	Putative uncharacterized protein	662
Q3TVI6	Dip3 beta	662
Q3U3Y3	Putative uncharacterized protein (Fragment)	405

Pfam Domains

DUF3263; PH; Pfam-B_3451; PID

Mouse alleles (2)

Allele Name	Allele Type
Appl2^{tm1a(KOMP)Wtsi}	KOMP-CSD-Project
Appl2^{tm1e(KOMP)Wtsi}	KOMP-CSD-Project

Human Homolog

APPL2 (adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2)

Human synonyms

DIP13B

Human cytoband

12q24.1

Known deafness gene

Within mapped deafness loci

DFNA25; DFNA41

Associated Human Disease in OMIM

None

Chicken homolog (1)

ENSGALG00000012665 (APPL2) encoding ENSGALP00000020645

Gene Ontology

Biological Process (3)	cell cycle; cell proliferation; cell proliferation
Cellular Component (6)	membrane; endosome; endosome membrane; cytoplasm; nucleus; nucleus
Molecular Function (2)	phospholipid binding; Rab GTPase binding

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	117	175	237	249	153	130
Cochlea GFP+ cells	53	150	467	484	569	NA
Utricle GFP- cells	95	185	158	139	183	582
Cochlea GFP- cells	109	118	181	395	354	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	227	GFP+	253	1.11	0.808
Utricle	200	Cochlea	288	1.44	1
Embryonic	125	Postnatal	306	2.44	0.415

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17243576	120.62 ± 7.19	129.97 ± 9.38 [131.06]	1.08	0.615

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	2928	2855	2663	2495
No bFGF	4640	4603	4181	3775
EGF	1759	1608	1461	1609

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	2495	No bFGF	3775	1.51	1
bFGF	2495	EGF	1609	0.64	6.46e-6
No bFGF	3775	EGF	1609	0.43	3.47e-21

Chicken Stereocilium Proteomics

Protein ID	ENSGALP00000020645
Symbol	APPL2
Description	Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2
log10 riBAQ in stereocilia (mean ± sd)	-4.59 ± 0.23
log10 riBAQ in utricle (mean ± sd)	-3.95 ± 0.21
Molecules per stereocilium (mean ± error with slope error)	37.98 ± 2.23
Corrected Molecules per stereocilium (mean ± sd)	4.77 ± 43.96
Stereocilium / Utricle ratio (mean ± sd)	0.23 ± 0.02
Enriched in the stereocilia (FDR < 0.05)	FALSE

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1426743_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	2997.97	2442.09	2981.59	3147.4	2694.27	4130.61
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	3005.72	3207.52	2450.14	3963.07	3151.2	2756.27
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	3228.41	3312.7	2343.17	2541.38	3569.7	2758.54
Replicate 3 Call	P	P	P	P	P	P
Replicate 4			2532.22	2459.11	3001.6	3033.98
Replicate 4 Call			P	P	P	P
Mean	3077.37	2987.44	2576.78	3027.74	3104.19	3169.85
Standard Deviation	130.86	475.2	280.75	694.99	363.99	653.64
Log2 Mean	11.59	11.53	11.33	11.54	11.59	11.61
Log2 Standard Deviation	0.06	0.24	0.15	0.32	0.17	0.28

Vestibular ganglion
Replicate 1	3159.26	3093.49	3108.69	3377.39	3525.51	3950.81
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	3028.34	3255.77	3433.14	2555.63	4153.66	3421.21
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	2982.34	3009.31	2836.72	3042.27	3871.14	3907.12
Replicate 3 Call	P	P	P	P	P	P
Mean	3056.65	3119.52	3126.18	2991.76	3850.1	3759.71
Standard Deviation	91.79	125.28	298.59	413.2	314.6	293.97
Log2 Mean	11.58	11.61	11.61	11.54	11.91	11.87
Log2 Standard Deviation	0.04	0.06	0.14	0.2	0.12	0.12

GENE VIEW

Appl2