SHIELD | Shared Harvard Inner-Ear Laboratory Database

MOUSE GENE INFORMATION [PubMed]

Description

aminolevulinate, delta-, dehydratase

Synonyms

Chromosome

Genetic Map Distance

33.17 cM

RefSeq Accessions

NM_008525; NP_032551

NCBI Entrez Gene

17025 at chr4:62170204-62181097 (mm9) / chr4:62505984-62520063 (mm10)

Ensembl Gene

ENSMUSG00000028393

VEGA Gene

OTTMUSG00000009022

MGI Gene

MGI:96853

Mouse Protein (4)

UniProt ID	Description	Length (AA)
P10518	Delta-aminolevulinic acid dehydratase	330
O89061	Delta-aminolevulinic acid dehydratase (Fragment)	203
Q8VCZ1	Alad protein	191
Q9DD05	Putative uncharacterized protein	330

Pfam Domains

ALAD; DUF2016

Mouse alleles (4)

Allele Name	Allele Type
Alad^{tm2a(EUCOMM)Wtsi}	EUCOMM-projects
Alad^{tm1a(EUCOMM)Wtsi}	EUCOMM-projects
Alad^{tm1e(EUCOMM)Wtsi}	EUCOMM-projects
Alad^{tm3a(EUCOMM)Wtsi}	EUCOMM-projects

Human Homolog

ALAD (aminolevulinate dehydratase)

Human synonyms

ALADH; PBGS

Human cytoband

9q33.1

Known deafness gene

Within mapped deafness loci

Associated Human Disease in OMIM

Porphyria, Acute Hepatic [Alad Deficiency|Delta-Aminolevulinate Dehydratase Deficiency|Porphobilinogen Synthase Deficiency|Porphyria, Alad]

Chicken homolog (1)

ENSGALG00000008869 (ALAD) encoding ENSGALP00000014392

Gene Ontology

Biological Process (18)	protein homooligomerization; response to organic substance; response to metal ion; response to zinc ion; metabolic process; porphyrin-containing compound biosynthetic process; response to nutrient; response to chemical stimulus; response to drug; response to ethanol; response to arsenic-containing substance; heme biosynthetic process; heme biosynthetic process; heme biosynthetic process; heme biosynthetic process; heme biosynthetic process; response to cadmium ion; tetrapyrrole biosynthetic process
Cellular Component (2)	extracellular space; cytosol
Molecular Function (14)	catalytic activity; catalytic activity; lyase activity; zinc ion binding; porphobilinogen synthase activity; porphobilinogen synthase activity; porphobilinogen synthase activity; porphobilinogen synthase activity; porphobilinogen synthase activity; metal ion binding; metal ion binding; identical protein binding; identical protein binding; lead ion binding

FACS-Sorted Hair Cells

Data | Chart

	E16a	E16b	P0	P4	P7	P16
Utricle GFP+ cells	34	5	19	18	33	45
Cochlea GFP+ cells	32	43	23	60	51	NA
Utricle GFP- cells	62	17	43	439	153	0
Cochlea GFP- cells	71	28	56	162	72	NA

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	100	GFP+	33	0.33	0.506
Utricle	72	Cochlea	60	0.83	1
Embryonic	36	Postnatal	84	2.28	0.951

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17426206	260.37 ± 15.68	183.18 ± 30.08 [221.56]	0.70	0.49

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	792	801	914	888
No bFGF	630	689	750	857
EGF	547	592	630	590

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	888	No bFGF	857	0.97	1
bFGF	888	EGF	590	0.66	0.000238
No bFGF	857	EGF	590	0.69	0.000636

Chicken Stereocilium Proteomics

Protein ID	ENSGALP00000014392
Symbol	ALAD
Description	Aminolevulinate dehydratase
log10 riBAQ in stereocilia (mean ± sd)	-4.69 ± 0.03
log10 riBAQ in utricle (mean ± sd)	-4.04 ± 0.34
Molecules per stereocilium (mean ± error with slope error)	30.27 ± 0.92
Corrected Molecules per stereocilium (mean ± sd)	3.26 ± 34.89
Stereocilium / Utricle ratio (mean ± sd)	0.22 ± 0.02
Enriched in the stereocilia (FDR < 0.05)	NA

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1424877_a_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	541.47	675.7	839.36	1718.34	1392.78	1357.25
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	599.69	731.5	1125.1	1370.62	2039.03	1439.58
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	676.64	787.37	1453.45	1251.93	1660.63	1752.51
Replicate 3 Call	P	P	P	P	P	P
Replicate 4			1008.02	1001.24	1570.43	1977.4
Replicate 4 Call			P	P	P	P
Mean	605.93	731.52	1106.48	1335.53	1665.72	1631.69
Standard Deviation	67.8	55.84	259.35	298.06	272.62	286.55
Log2 Mean	9.24	9.51	10.08	10.36	10.69	10.66
Log2 Standard Deviation	0.16	0.11	0.33	0.32	0.23	0.25

Vestibular ganglion
Replicate 1	607.57	960.94	977.59	1079.68	1142.04	1523.06
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	607.96	918.56	1275.11	1103	1043.62	1414.78
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	630.34	840.08	793.16	1140.89	1314.13	1485.77
Replicate 3 Call	P	P	P	P	P	P
Mean	615.29	906.53	1015.29	1107.86	1166.6	1474.54
Standard Deviation	13.04	61.32	243.18	30.89	136.92	55.01
Log2 Mean	9.26	9.82	9.96	10.11	10.18	10.53
Log2 Standard Deviation	0.03	0.1	0.34	0.04	0.17	0.05

GENE VIEW

Alad