SHIELD | Shared Harvard Inner-Ear Laboratory Database

GENE VIEW

Abcb1a

MOUSE GENE INFORMATION [PubMed]

Description

ATP-binding cassette, sub-family B (MDR/TAP), member 1A

Synonyms

Pgy-3; Pgp; Pgy3; MDR3; P-gp; Evi32; mdr-3; P-glycoprotein; Mdr1a; multiple drug resistant 1a

Chromosome

Genetic Map Distance

3.43 cM

RefSeq Accessions

NM_011076; NP_035206

NCBI Entrez Gene

18671 at chr5:8660092-8748570 (mm9) / chr5:8567090-8748575 (mm10)

Ensembl Gene

ENSMUSG00000040584

VEGA Gene

OTTMUSG00000029053

MGI Gene

MGI:97570

Mouse Protein (4)

UniProt ID	Description	Length (AA)	Predicted # TMs
P21447	Multidrug resistance protein 3	1276	11
Q3UMH0	Putative uncharacterized protein	490	3
Q9CVG4	Putative uncharacterized protein (Fragment)	19	0
Q9QX25	P-glycoprotein (Fragment)	52	0

Pfam Domains

Mouse alleles (4)

Allele Name	Allele Type
Abcb1a^mds	Spontaneous
Abcb1a^tm1.1Kane	Targeted (Reporter)
Abcb1a^tm1Bor	Targeted (knock-out)
Abcb1a^tm1Kane	Targeted (Reporter)

Human Homolog

ABCB1 (ATP-binding cassette, sub-family B (MDR/TAP), member 1)

Human synonyms

ABC20; CD243; CLCS; GP170; MDR1; P-GP; PGY1

Human cytoband

7q21.12

Known deafness gene

Within mapped deafness loci

Associated Human Disease in OMIM

Niemann-Pick Disease, Type C1; NPC1 [Niemann-Pick Disease with Cholesterol Esterification Block|Niemann-Pick Disease without Sphingomyelinase Deficiency|Niemann-Pick Disease, Chronic Neuronopathic Form|Niemann-Pick Disease, Subacute Juvenile Form|Niemann-Pick Disease, Type C; NPC]

Inflammatory Bowel Disease 13; IBD13

Chicken homolog (4)

ENSGALG00000008912 (O93437_CHICK) encoding ENSGALP00000014477

ENSGALG00000008912 (O93437_CHICK) encoding ENSGALP00000037427

ENSGALG00000023271 (ABCB1) encoding ENSGALP00000014451

ENSGALG00000023271 (ABCB1) encoding ENSGALP00000037453

Gene Ontology

Biological Process (9)	transmembrane transport; stem cell proliferation; negative regulation of cell death; drug transmembrane transport; G2/M transition of mitotic cell cycle; transport; transport; ATP catabolic process; drug transport
Cellular Component (7)	apical plasma membrane; integral to membrane; Golgi membrane; cell surface; membrane; plasma membrane; intercellular canaliculus
Molecular Function (10)	ATPase activity; coupled; ATPase activity; coupled to transmembrane movement of substances; xenobiotic-transporting ATPase activity; nucleoside-triphosphatase activity; ATPase activity; hydrolase activity; ATP binding; ATP binding; nucleotide binding; nucleotide binding

FACS-Sorted Hair Cells

Data | Chart

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
GFP-	5	GFP+	1	0.23	0.402
Utricle	3	Cochlea	2	0.82	1
Embryonic	2	Postnatal	3	1.46	0.993

Adult Cochlear Inner and Outer Hair Cells

Probeset ID	IHC (mean ± sd)	OHC (mean ± sd) [OHC replicate]	Fold Change (OHC/IHC)	False Discovery Rate (FDR)
17434524	17.22 ± 2.13	23.49 ± 5.99 [29.39]	1.36	0.552

Otic Progenitor Cells

Data

Growth Factor	Replicate 1	Replicate 2	Replicate 3	Mean
bFGF	29	17	19	160
No bFGF	10	6	14	9
EGF	1	4	8	4

Analysis

Condition A	Mean A	Condition B	Mean B	Fold Change (B/A)	False Discovery Rate (FDR)
bFGF	160	No bFGF	9	0.06	1.84e-8
bFGF	160	EGF	4	0.03	2.99e-58
No bFGF	9	EGF	4	0.51	0.125

Chicken Stereocilium Proteomics

The chicken protein was not reproducibly detected in the chicken stereocilia.

The chicken protein was not reproducibly detected in the chicken stereocilia.

Auditory and Vestibular Ganglion Neurons

Probe set ID: 1419758_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	13.75	20.65	74	320.48	1001.89	187.44
Replicate 1 Call	A	A	P	P	P	P
Replicate 2	16.59	15.03	111.47	191.5	1274.8	174.11
Replicate 2 Call	A	A	P	P	P	P
Replicate 3	31.04	11.06	99.38	83.94	891.66	217.47
Replicate 3 Call	A	A	P	P	P	P
Replicate 4			340.83	130.52	1088.54	377.88
Replicate 4 Call			P	P	P	P
Mean	20.46	15.58	156.42	181.61	1064.22	239.23
Standard Deviation	9.27	4.82	123.93	102.52	161.86	94.2
Log2 Mean	4.26	3.92	7.01	7.33	10.04	7.83
Log2 Standard Deviation	0.62	0.45	0.96	0.82	0.22	0.51

Vestibular ganglion
Replicate 1	42.98	54.17	873.51	860.09	1675.48	1916.37
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	45.74	53.95	681.68	671.12	1381.94	1806.28
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	32.17	78.69	1129.72	590.79	1642.64	1833.57
Replicate 3 Call	A	P	P	P	P	P
Mean	40.3	62.27	894.97	707.33	1566.69	1852.07
Standard Deviation	7.17	14.22	224.79	138.25	160.84	57.33
Log2 Mean	5.32	5.94	9.78	9.45	10.61	10.85
Log2 Standard Deviation	0.27	0.31	0.36	0.28	0.15	0.04

Probe set ID: 1419759_at

Age	E12	E13	E16	P0	P6	P15
Spiral ganglion
Replicate 1	29.78	37.37	240.76	806.7	2144.07	802.5
Replicate 1 Call	A	P	P	P	P	P
Replicate 2	22.93	29.87	234.13	460.38	3125.9	949.43
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	27.71	29.36	296.29	340.64	1945.95	1109.07
Replicate 3 Call	A	P	P	P	P	P
Replicate 4			641.05	440.22	1927.33	1276.98
Replicate 4 Call			P	P	P	P
Mean	26.81	32.2	353.06	511.99	2285.81	1034.5
Standard Deviation	3.51	4.48	194.01	203.33	568.58	204.47
Log2 Mean	4.74	5	8.33	8.92	11.13	9.99
Log2 Standard Deviation	0.19	0.19	0.68	0.52	0.33	0.29

Vestibular ganglion
Replicate 1	48.24	132.71	1723.64	2145.47	2615.5	3135.7
Replicate 1 Call	P	P	P	P	P	P
Replicate 2	49.94	186.42	1497.66	1555.15	2540.5	3078.01
Replicate 2 Call	P	P	P	P	P	P
Replicate 3	39.01	194.48	1746.08	1105.98	2367.5	3359.54
Replicate 3 Call	P	P	P	P	P	P
Mean	45.73	171.2	1655.79	1602.2	2507.83	3191.08
Standard Deviation	5.88	33.58	137.41	521.34	127.19	148.71
Log2 Mean	5.51	7.4	10.69	10.59	11.29	11.64
Log2 Standard Deviation	0.19	0.3	0.12	0.48	0.07	0.07

Utricle GFP+ cells

Cochlea GFP+ cells

Utricle GFP- cells

Cochlea GFP- cells